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Here are the 49 US AI companies that have raised $100M in 2025

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Here are the 49 US AI companies that have raised $100M in 2025

U.S. AI Startups Surpass $100 Million Funding Milestones Throughout 2025

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The artificial intelligence sector in the United States and globally has experienced unprecedented growth in 2025. According to recent data, the number of AI startups securing funding rounds exceeding $100 million has already matched last year’s total, with a notable increase in companies achieving multiple mega-rounds this year.

In 2024, 49 AI startups raised $100 million or more, with seven surpassing the $1 billion mark in single funding rounds. This momentum has accelerated in 2025, reflecting growing investor confidence and the expanding applications of AI technologies across industries.

Highlights of November 2025 Funding Rounds

  • Anysphere, the creator of the popular AI coding platform Cursor, secured a massive $2.3 billion in its second funding round this year, pushing its valuation to $29.3 billion as announced on November 13.
  • Parallel, specializing in web infrastructure for AI agents, closed a $100 million Series A round on November 12, led by Index Ventures and Kleiner Perkins.
  • Hippocratic AI, focused on healthcare AI agents, raised $126 million in a Series C round on November 3, valuing the company at $3 billion. This was their second funding round in 2025, led by Avenir Growth.

October 2025: A Surge in AI Investment

  • Fireworks AI, a platform enabling users to build AI applications with open-source models, announced a $250 million Series C round on October 28, valuing the company at $4 billion.
  • Uniphore, an enterprise AI startup, raised $260 million in a Series F round on October 22, reaching a $2.5 billion valuation. Notable investors included Snowflake Ventures, Nvidia, Databricks Ventures, and AMD.
  • Sesame, a voice-AI company, secured $250 million in Series B funding led by Sequoia Capital and Spark Capital on October 21, with participation from SignalRank.
  • OpenEvidence, based in Cambridge, MA, raised $200 million in a Series C round on October 20, achieving a $6 billion valuation.
  • Lila Sciences, aiming to develop a superintelligence platform for scientific research, closed a $350 million Series A round on October 14, led by Braidwell and Collective Global.
  • DeepSeek announced a $2 billion Series B round on October 9, led by Nvidia, valuing the company at $8 billion.
  • EvenUp, which develops AI solutions for personal injury law, raised $150 million in a Series E round on October 7, valuing the company above $2 billion. Bessemer led the round with support from Lightspeed, Bain Capital, and SignalFire.

September 2025: Expanding AI Horizons

  • Periodic Labs, developing AI-driven scientific research tools, announced a $300 million seed round on September 30, with investors including Felicis, Andreessen Horowitz, Lightspeed, and Khosla Ventures.
  • Cerebras Systems, an AI infrastructure leader, raised $1.1 billion in a Series G round on September 30, valuing the company at $8 billion. The round was led by Atreides Management and Fidelity.
  • Modular secured $250 million on September 24 to scale its unified AI compute platform, with US Innovative Technology Fund leading and participation from GV, Greylock, and General Catalyst.
  • Distyl AI, focused on enterprise AI software, raised $175 million in a Series B round on September 23, valuing the startup at $1.8 billion. Khosla Ventures was a key investor.
  • Upscale AI, an AI infrastructure startup, closed a $100 million seed round on September 17, co-led by Maverick Silicon and Mayfield, with Qualcomm Ventures and Stanford University among participants.
  • Groq, specializing in AI inference hardware, raised $750 million in a Series E round on September 17, reaching a valuation near $6.9 billion.
  • Invisible Technologies, an AI training company, raised $100 million on September 16, led by Vanara Capital, with Greycroft and Tallwoods Capital also investing, valuing the company at $2 billion.
  • Cognition AI, creators of the vibe-coding agent Devin, secured $400 million in a Series C round on September 8, led by Founders Fund, with a valuation of $10.2 billion.
  • Baseten, an AI infrastructure provider, raised $150 million in a Series D round on September 5, led by Bond Capital, with CapitalG, IVP, and Spark Capital participating, valuing the company at $2.1 billion.
  • Sierra raised $350 million on September 4, led by Greenoaks Capital, with a valuation exceeding $10 billion.
  • You.com secured $1.4 billion for its personalized AI search engine, with a $100 million Series C round led by Cox Enterprises announced on September 3, valuing the company at $1.5 billion.
  • Anthropic, an AI research lab, raised $13 billion in a Series F round on September 2, led by Iconiq, Fidelity, and Lightspeed, valuing the company at approximately $183 billion.

August 2025: Notable AI Funding Events

  • EliseAI, a healthcare and housing automation platform, raised $250 million in a Series E round led by Andreessen Horowitz, valuing the company at $2.2 billion (announced August 20).
  • Decart, an AI research lab, secured $100 million at a $3.1 billion valuation on August 7, with Sequoia Capital and Benchmark Ventures among investors.

Mid-Year 2025 Funding Highlights

  • Fal, a generative media platform, raised $125 million in a Series C round led by Meritech Capital Partners on July 31, valuing the company at $1.5 billion. Salesforce Ventures, Shopify Ventures, and Google AI Futures Fund also participated.
  • Ambience Healthcare, developing an AI healthcare operating system, closed a $243 million Series C round led by Oak HC/FT and Andreessen Horowitz, with Kleiner Perkins and OpenAI Startup Fund joining (announced July).
  • Reka AI, an AI research lab, raised $110 million in a Series B round on July 22, with Snowflake and Nvidia as key investors, valuing the company at $1 billion.
  • Thinking Machines Lab secured $2 billion in a large seed round on July 15, led by Andreessen Horowitz, Nvidia, Accel, and AMD, valuing the company at approximately $12 billion.
  • OpenEvidence raised $210 million in a Series B round on July 15, led by Kleiner Perkins and GV, with a valuation of $3.5 billion.
  • Harmonic, developing mathematical reasoning AI, closed a $100 million Series B round led by Kleiner Perkins on July 10, with a valuation near $875 million.

June 2025: Healthcare and Legal AI Innovations

  • June, a healthcare AI unicorn, raised $300 million in a Series E round led by Andreessen Horowitz and Khosla Ventures, valuing the company at $5.3 billion.
  • Harvey, an AI legal tech startup, announced a $300 million Series E round on June 23, led by Kleiner Perkins and Coatue, with a valuation of $5 billion.
  • Tennr, a healthcare AI startup, raised $101 million in a Series C round led by IVP, with participation from Lightspeed, GV, and Andreessen Horowitz, valuing the company at $605 million.
  • Glean, an enterprise search startup, secured $150 million in a Series F round led by Wellington Management on June 10, with Sequoia, Lightspeed, and Kleiner Perkins participating, valuing the company at $7.25 billion.
  • Anysphere raised $900 million in a Series C round led by Thrive Capital, Andreessen Horowitz, and Accel, valuing the AI research lab at nearly $10 billion.

May 2025: Emerging AI Infrastructure and Benchmarking Tools

  • Snorkel AI, specializing in AI data labeling, raised $100 million in a Series D round led by Addition on May 29, with Prosperity7 Ventures and Lightspeed joining, valuing the company at $1.3 billion.
  • LMArena, a community-driven AI benchmarking platform, secured $100 million in a seed round on May 21, led by Andreessen Horowitz and UC Investments, with Lightspeed, Kleiner Perkins, and Felicis participating, valuing the startup at $650 million.
  • TensorWave, an AI infrastructure company based in Las Vegas, closed a $100 million Series A round on May 14, led by Magnetar Capital, with AMD Ventures, Nexus Venture Partners, and Maverick Silicon also investing.

Spring 2025: Major AI Funding Announcements

  • SandboxAQ raised $450 million in a Series E round on April 4, valuing the AI model company at $5.7 billion. Investors included Nvidia, Google, and Bridgewater Associates founder Ray Dalio.
  • Runway, an AI media production platform, secured $308 million in a Series D round on April 3, led by General Atlantic, with SoftBank, Nvidia, and Fidelity participating, valuing the company at $3 billion.
  • OpenAI closed a record-breaking $40 billion funding round on March 31, led by SoftBank, valuing the company at $300 billion. Thrive Capital and Microsoft were also key investors.
  • Nexthop AI announced a $110 million Series A round on March 25, led by Lightspeed Venture Partners, with Kleiner Perkins, Battery Ventures, and Emergent Ventures participating.
  • Insilico Medicine, focused on AI-driven drug discovery, raised $110 million in a Series E round on March 13, led by Value Partners and Pudong Chuangtou, valuing the company at $1 billion.
  • Celestial AI, an AI infrastructure startup, secured $250 million in a Series C round on March 11, led by Fidelity, with Tiger Global, BlackRock, and Intel CEO Lip Bu Tan investing, valuing the company at $2.5 billion.
  • Lila Sciences raised $200 million in a seed round led by Flagship Pioneering, aiming to build a superintelligence platform for scientific discovery.
  • Reflection AI, based in Brooklyn, raised $130 million in a Series A round led by Lightspeed Venture Partners and CRV, valuing the company at $580 million.
  • Turing, an AI coding startup, completed a $111 million Series E round on March 7, valuing the company at $2.2 billion. Khazanah Nasional led the round with WestBridge Capital, Gaingels, and Sozo Ventures participating.
  • Shield AI, specializing in AI defense technology, raised $240 million in a Series F round on March 6, valuing the company at $5.3 billion.
  • Anthropic raised $3.5 billion in a Series E round on March 3, led by Lightspeed, with Salesforce Ventures, Menlo Ventures, and General Catalyst participating, valuing the company at $61.5 billion.

February 2025: Growth in Open-Source and Legal AI

  • Together AI, an open-source generative AI and model development platform, raised $305 million in a Series B round on February 20, led by Prosperity7 and General Catalyst, with Salesforce Ventures, Nvidia, and Lux Capital also investing. The company’s valuation reached $3.3 billion.
  • Lambda, an AI infrastructure provider, closed a $480 million Series D round on February 19, led by SGW and Andra Capital, with Nvidia, G Squared, and ARK Invest participating, valuing the startup at $2.5 billion.
  • Abridge, an AI platform transcribing patient-clinician conversations, raised $250 million in a Series D round on February 17, led by IVP and Elad Gill, with Lightspeed, Redpoint, and Spark Capital also involved, valuing the company at $2.75 billion.
  • Eudia, an AI legal tech firm, secured $105 million in a Series A round on February 13, led by General Catalyst, with Floodgate, Defy Ventures, and Everywhere Ventures participating.
  • EnCharge AI, an AI hardware startup founded in 2022, raised $100 million in a Series B round on February 13, led by Tiger Global, with Scout Ventures and Samsung Ventures joining.
  • Harvey, an AI legal tech company, raised $300 million in a Series D round on February 12, led by Sequoia Capital, valuing the three-year-old startup at $3 billion.

January 2025: Early Year Funding Highlights

  • ElevenLabs, a synthetic voice technology startup, raised $180 million in a Series C round on January 30, co-led by ICONIQ Growth and Andreessen Horowitz, with Sequoia Ventures, NEA Ventures, and Salesforce Ventures also participating, valuing the company at over $3 billion.
  • Hippocratic AI, developing large language models for healthcare, announced a $141 million Series B round in January, led by Kleiner Perkins, with Andreessen Horowitz, Nvidia, and General Catalyst also investing, valuing the company at over $1.6 billion.

Stay updated with the latest AI funding news and trends as the industry continues to evolve rapidly in 2025.

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